Coding

Part:BBa_K3972001:Design

Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen   Group: iGEM21_TU-Eindhoven   (2021-09-20)


TtrR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 317
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 317
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 317
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 317
    Illegal AgeI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Only Type II S BioBrick compatible.


Source

From plasmid pKD223.7-3 ordered from Addgene.

References