Coding
Part:BBa_K3972001:Design
Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen Group: iGEM21_TU-Eindhoven (2021-09-20)
TtrR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 317
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 317
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 317
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 317
Illegal AgeI site found at 247 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Only Type II S BioBrick compatible.
Source
From plasmid pKD223.7-3 ordered from Addgene.